Fluo-2 High Affinity AM Documentation

Fluo-2 AM High Affinity Product Specifications

Cell-permeable acetoxymethyl (AM) ester derivative of Fluo-2 HA. Approximately twice as bright as Fluo-4, four times Fluo-3.

Molecular Weight

1061 g/mol

Kd

230 nM

Odor

None

Fire and Explosion Hazards

None

Toxicology Data

Not known

Handling and Storage

Store at -20°C. Protect from light and moisture

Shelf Life

Valid for six months after delivery, if stored properly

TLC

Solvent

Ethyl Acetate / Hexane 2:1

Rf

0.5

HPLC

Column

C18

Detector Settings

254 nm, 371 nm

Purity

> 95%

Absorbance Spectrum

Solvent

Ethyl Acetate

Absorbance max

455 nm

Fluorescence Spectrum

Fluorescence after hydrolized

Solvent

Ethyl Acetate

Excitation max

490 nm

Emission max

516 nm

1H NMR

All relevant peaks present

Solvent

Deuterated chloroform

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Fluo-2 AM High Affinity Background

Fluo-2_HighAff_AM_Background

Most useful and inexpensive lasers operate in the green part of the visible spectrum and that has made green-emitting fluorescent calcium indicators very useful for confocal microscopy, flow cytometry, and screening. The popular green-emitting fluorescent calcium indicators for screening are Fluo-2, Fluo-3, Fluo-4, and Fluo-8. They are all derived from the original Fluo compound invented by Minta and Tsien (1989).

Fluo-2 is one of the original Fluo series of Ca2+ indicators invented by Roger Tsien in the 1980s. Whereas Fluo-3 and Fluo-4 were commercialized, Fluo-2 was not. Subsequently, Fluo-2 has been found to be much brighter (1.5x) than Fluo-4 in cellular experiments. The increased brightness of Fluo-2 is partly attributable to its superior loading in most cell types.

The patent for Fluo-2, Fluo-3, and Fluo-4 have expired so Ion Indicators offers these at reasonable prices.

Dye Characteristic Comparison

Reagents for viability and Toxicity Assays

MW (g/mol)

Excitation (nm)

Emission (nm)

Kd

Extinction

Solubilty

Fluo-2 AM

1061

N/A

N/A

N/A

26,000

DMSO

Fluo-2 K+ Salt

891

490

494

230

80,000

H2O

Fluo-3 AM

1130

N/A

N/A

N/A

26,000

DMSO

Fluo-3 K+ Salt

960

506

515

390

80,000

H2O

Fluo-4 AM

1097

N/A

N/A

N/A

26,000

DMSO

Fluo-4 K+ Salt

927

490

526

345

80,000

H2O

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Buy Fluo-2 AM High Affinity

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