Fura-2 AM Documentation

Fura-2 AM Product Specifications

Cell-permeable acetoxymethyl (AM) ester derivative of Fura-2, a UV-excitable, excitation-ratiometric calcium indicator.

Molecular Weight

1002 g/mol

Solubility

DMSO

Kd

145 nM

Fluorescence changes due to calcium binding are observed only after hydrolysis of AM esters by non-specific esterases to the Fura-2 pentanion.

Odor

None

Fire and Explosion Hazards

None

Toxicology Data

Not known

Handling and Storage

Store at -20°C. Protect from light and moisture

Shelf Life

Valid for one year after delivery, if stored properly

TLC

Solvent

2:1 Ethyl Acetate/Hexanes

Rf

0.5

HPLC

Column

C18

Detector Settings

254 nm, 371 nm

Purity

> 95%

Absorbance Spectrum

Solvent

Ethyl Acetate

Absorbance max

371 ± 3 nm

ε

33000 M-1cm-1

Fluorescence Spectrum

Solvent

Ethyl Acetate

Excitation max

371 nm ± 3 nm

Emission max

471 nm ± 3 nm

Mass Spectrum

Electrospray Ionization

Mass-to-Charge Ratio

1002 M/Z

1H NMR

All relevant peaks present

Solvent

Deuterated acetone

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Fura-2 AM Results

Fura-2 is one of the first commercial fluorescent calcium indicators introduced by Dr. Tsien and produced by Molecular Probes in 1986. It is now so common in the field that it appears in standard molecular cell biology textbooks.
UV-excitable Calcium Indicators
Catalog Number MW (g/mol) Absorbance (nm) Excitation (nm) Emission (nm) Kda (nm) Solubility
Fura-2 AM 1051B
1051E
1002
340/380 nm UV
371 N/A* N/A* N/A* DMSO
Fura-2 K+ Salt 1052B
1052E
832 354 340/380
(high/low CA2+)
505 145 H20

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Buy Fura-2 AM

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