ION Potassium Green-2 AM Documentation

ION Potassium Green-2 AM Product Specifications

Molecular Weight

1128 g/mol

Solubility

DMSO

Kd

18 nm

Odor

None

Fire and Explosion Hazards

None

Toxicology Data

Not known

Handling and Storage

Store at -20°C; protect from light and moisture

Shelf Life

Valid for one year after delivery, if stored properly


TLC

Solvent

7% Methanol/ Chloroform

Rf

0.6

HPLC

Column

C18

Detector Settings

254 nm and 460 nm

Purity

> 80%

Absorbance Spectrum

Solvent

Methanol

Absorbance max

469± 3 nm

ε

21,000 M-1cm-1

FluorescenceSpectrum

Solvent

Methanol

Emission max

546 ± 3 nm

Excitation max

526 ± 3 nm

11H NMR

All relevant peaks present

Solvent

Deuterated acetone

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ION Potassium Green-2 AM Background

ION_Potassium_Green-2_AM_Theory

The importance of the potassium ion (K+) being coupled to the sodium ion (Na+) is that the cell expends a major part of its metabolic energy maintaining the concentrations of Na+ and K+ within the cell. Intracellular concentration ranges are 10-40 mM for Na+ and 120-400 mM for K+. Extracellular concentration ranges are 4-40 mM for K+ and 120-400 mM for Na+.

In the absence of a K+ indicator, efforts have been directed to using indirect techniques to measure or detect K+, including

  • Analogs like thallium or cesium to monitor K+ fluxes
  • The pH indicator BCECF AM and the ionophore nigericin in flow cytometry studies
  • Combinations of ion selective carriers
  • Ion-channel mediated K+ fluxes with membrane potential changes registered by voltage sensitive dyes
  • Fiber-optic sensors for K+ with pH sensitive dyes

These alternate techniques were necessary because the previously reported fluorescent potassium indicator PBFI requires UV excitation and suffers from poor loading and poor brightness. Asante Potassium Green 1 and 2 (APG-1 and APG-2) successfully address these problems.

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ION Potassium Green-2 AM Results

Figure 1 ION Potassium Green-2 Emission Titration

Ion Potassium Green-2 Emission Titration

Figure 2 ION Potassium Green-1 Emission Titration

ION Potassium Green-1 Emission Titration

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ION Potassium Green-2 AM Comparison

ION_Potassium_Green-2_AM_Comparison

ION Potassium Green and PBFI

PBFI

IPG-1/IPG-2

Kd

5nM

54nM/18nM

Excitation

340 and 390 nm

488-517 nm

Emission

500 nm

540 nm

Brightness

Dim

Very Bright

Dynamic Range (Fmax/Fmin)

<3

12

Photo-bleaching

Sensitive

Very Stable

AM ester loading

Difficult

Easy

*Products only available via special request.

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Buy ION Potassium Green-2 AM

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