ION NaTRIUM Green-2 TMA+ Salt Documentation

ION NaTRIUM Green-2 TMA+ Salt Product Specifications

Molecular Weight

1087 g/mol

Solubility

H2O

Kd

20 nM (in absence of K+)

Odor

None

Fire and Explosion Hazards

None

Toxicology Data

Not known

Handling and Storage

Store at -20°C. Protect from light and moisture

Shelf Life

Valid for one year after delivery, if stored properly

TLC on Reverse Phase Plate

Solvent

7:3 Methanol/3M Sodium Acetate

Rf

0.5

HPLC

Column

C18

Detector Settings

254 nm, 475 nm2

Purity

> 90%

Absorbance Spectrum

Solvent

Methanol

Absorbance max

523 ± 3 nm

ε

5000 M-1cm-1

Fluorescence Spectrum

Solvent

135 mM NaCl, 10 mM MOPS, pH 7.2

Emission max

545 nm

Excitation max

525 nm

11H NMR

All relevant peaks present

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ION Natrium Green-2 TMA+ Salt Background

There is a large difference in the sodium ion (Na+) concentration inside and outside the cell (5-40 mM intracellular; 120-450 mM extracellular, depending on organism). This concentration gradient is used to power nutrient uptake, to regulate concentrations of other intracellular ions and solutes, and to generate and transmit electrical impulses in excitable cells such as nerve and muscle. These functions are so important that organisms devote a major part of their metabolic energy to maintaining the sodium gradient. The low intracellular Na+ concentration requires that a Na+ indicator have the sensitivity to measure any small changes that occur. Moreover, intracellular potassium ion (K+) concentration is typically high (in excess of 100 mM); therefore a Na+ indicator should respond selectively to Na+, not K+.

ING-2 is a higher affinity analog of ING-1, as demonstrated by comparing the fluorimetric emission sodium chloride titrations of ING-1 (Figure 1) and ING-2 (Figure 2), with identical excitation and emission wavelengths. It retains the desirable properties of easy loading, slow leakage, and good photostability.

Figure 1

ION Natrium Green-1 Emission Spectra

ION Natrium Green-2 TMA+ Salt Background

Figure 2

ION Natrium Green-2 Emission Spectra

ION Natrium Green-2 TMA+ Salt Background

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Buy ION NaTRIUM Green™-2 TMA+ Salt

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