SBFI AM Documentation

SBFI AM Product Specifications

SBFI is an ultraviolet (340 and 380 nm) excitation ratiometric Na+ indicator.

Molecular Weight

1127.07g/mol

Solubility

DMSO

Kd

4 nM

Physical Description

Yellow solid

Odor

None

Fire and Explosion Hazards

None

Toxicology Data

Not known

Handling and Storage

Store at -20°C. Protect from light and moisture

Shelf Life

Valid for one year after delivery, if stored properly

TLC

Solvent

10% methanol/chloroform

Rf

0.35

HPLC

Gradient

40-100%

Column

MeCN/50 mM TEA-Ac at pH 7.0

Detector Settings

C2, packed in SiO2

Purity

> 90%

Absorbance Spectrum

Solvent

MeOH

Absorbance max

379 nm

ε

32000 M-1cm-1

Excitation Spectrum

Solvent

pH 7 buffer

Excitation max

348 nm (low ion) after AM ester hydrolysis

Excitation max

335 nm (high ion) after AM ester hydrolysis

1Emission Spectrum

Solvent

pH 7 buffer

Emission max

510 nm (low ion)

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SBFI AM Results

SBFI_AM_Results

SBFI

ING-1*/ING-2

Kd

4 mM

92 nM/20nM

Excitation

340 and 380 nm

488 to 517 nm

Emission

500 nm

540 nm

Brightness

Dim

Very Bright

Dynamic Range (Fmax/Fmin)

<3

29

AM ester loading

Difficult

Easy

Photobleaching

Sensitive

Resistant

*Products only available via special request.

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Buy SBFI AM

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