SBFI K+ Salt Documentation

SBFI K+ Salt Results

SBFI_K+_Salt_Results

Ion NaTRIUM Green Dye Characteristic Comparison

SBFI

ING-1*/ING-2

Kd

4 mM

92 nM/20nM

Excitation

340 and 380 nm

488 to 517 nm

Emission

500 nm

540 nm

Brightness

Dim

Very Bright

Dynamic Range (Fmax/Fmin)

<3

29

AM ester loading

Difficult

Easy

Photobleaching

Sensitive

Resistant

*Products only available via special request.

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SBFI K+ Salt Product Specifications

SBFI is an ultraviolet (340 and 380 nm) excitation ratiometric Na+ indicator. Cell- Ratiometric, sodium sensitive dye, 18 times more selective for Na+ than K+.

Molecular Weight

991 g/mol

Solubility

Water (pH > 8)

Kd

3.9 nM

Physical Description

Yellow solid

Odor

None

Fire and Explosion Hazards

None

Toxicology Data

Not known

Handling and Storage

Store at -20°C. Protect from light and moisture

Shelf Life

Valid for one year after delivery, if stored properly

TLC on Reverse-Phase Plate

Solvent

7:3 methanol/water

Rf

0.9

HPLC

Column

C18

Detector Settings

254 nm and 336 nm

Purity

> 95%

Absorbance Spectrum

Solvent

135 mM TMACl, 10mM MOPS, pH 7.2

Absorbance max

339 nm ± 3 nm

ε

45000 M-1cm-1

Fluorescence Spectrum

Solvent

135mM TMACl, 10mM MOPS, pH 7.2

Excitation max

345 nm ± 3 nm

Emission max

544 nm ± 3 nm

1H NMR

All relevant peaks present

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